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lipofectin method  (Thermo Fisher)


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    Structured Review

    Thermo Fisher lipofectin method
    Lipofectin Method, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lipofectin+method/pm23921576-61-39-41
    Average 86 stars, based on 1 article reviews
    lipofectin method - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Transfection:

    Article Title: Conditional expression of PTEN alters the androgen responsiveness of prostate cancer cells.
    Article Snippet: Z. Wu, M. Conaway, D. Gioeli, M.J. Weber, and D. Theodorescu* DepartmentofMolecular PhysiologyandBiological Physics,Universityof VirginiaHealth Sciences Center, Charlottesville,Virginia DepartmentofHealth Evaluation Sciences (Biostatistics),Universityof VirginiaHealth SciencesCenter, Charlottesville,Virginia DepartmentofMicrobiology,Universityof VirginiaHealth Sciences Center,Charlottesville,Virginia

    Article Title: Method for regulating genes with electromagnetic response elements
    Article Snippet: .. Materials and Methods Cell Culture and Transfections As previously described, HeLa cells were used for transient transfections and the lipofectin method (Gibco/BRL, Cat # 18292-011) was used for transfection as described (6) (7) (8). ..

    Article Title: Differential Ras activation between caveolae/raft and non-raft microdomains.
    Article Snippet: .. Cell culture, transfection, and imaging COS cells were transfected with pRaichu-tH or pRaichu-tK using the Lipofectin method (Gibco BRL). .. For disruption of caveolae/ rafts, cells were incubated in Hank’s Balanced Salt Solution (HBSS) containing 10 mM methyl-β-cyclodextrin (MβCD) (Sigma-Aldrich) for 30 min at 37°C (Prior et al., 2003; Roy et al., 1999).

    Article Title: Bromodomain analysis of Brd2-dependent transcriptional activation of cyclin A
    Article Snippet: .. Low passage NIH/3T3 cells (A.T.C.C., Manassas, VA, U.S.A.) were grown in DMEM (Dulbecco's modified Eagle's medium; Invitrogen) supplemented with 10% normal calf serum and PSG (penicillin, streptomycin and glutamine), maintained at less than 75% confluence and transfected in triplicate in 60 mm dishes at 50% confluence with plasmids by the Lipofectin® method (Invitrogen), then harvested at 100% confluence after 24–36 h. Transfections contained 3.0 μg of DNA/dish, comprised of 1.0 μg of Brd2 construct, 0.5 μg of cyclin A promoter luciferase reporter and 0.5 μg of Ki-ras expression vector, and were balanced with empty pcDNA(I) vector (Invitrogen). .. Normalization of luciferase activity was initially performed with reference to units of expressed β-galactosidase from a co-transfected vector and, after high reproducibility had been established, performed against protein concentration in the cell lysates.

    Article Title: A novel double-enhanced suicide gene therapy in a colon cancer cell line mediated by gef and apoptin.
    Article Snippet: Background Double-suicide gene therapy is a promising strategy for the treatment of advanced cancer.. It has become an important research line in the development of gene therapy to overcome the drawbacks of single-gene therapy.. Objective The aim of this study was to investigate the usefulness of double-suicide gene therapy with the two suicide genes, gef and apoptin, in colon carcinoma.

    Article Title: A novel point mutation in the ligand-binding domain (LBD) of the human glucocorticoid receptor (hGR) causing generalized glucocorticoid resistance: the importance of the C terminus of hGR LBD in conferring transactivational activity.
    Article Snippet: .. CV-1, COS-7, and HCT-116 cells were transfected using the lipofectin method (Invitrogen Life Technologies, Inc., Gaithersburg, MD) as previously described (26). .. HeLa cells were transfected using FuGene 6 reagent according to the instructions of the manufacturer (Roche, Indianapolis, IN).

    other:

    Article Title: Novel codon-optimized GM-CSF gene as an adjuvant to enhance the immunity of a DNA vaccine against HIV-1 Gag.
    Article Snippet: NIH3T3, CT-26, and eLa cells were transfected by the lipofectin method as uggested by the manufacturer (Life Technologies, Gaithersurg, MD).

    Cell Culture:

    Article Title: Method for regulating genes with electromagnetic response elements
    Article Snippet: .. Materials and Methods Cell Culture and Transfections As previously described, HeLa cells were used for transient transfections and the lipofectin method (Gibco/BRL, Cat # 18292-011) was used for transfection as described (6) (7) (8). ..

    Article Title: Differential Ras activation between caveolae/raft and non-raft microdomains.
    Article Snippet: .. Cell culture, transfection, and imaging COS cells were transfected with pRaichu-tH or pRaichu-tK using the Lipofectin method (Gibco BRL). .. For disruption of caveolae/ rafts, cells were incubated in Hank’s Balanced Salt Solution (HBSS) containing 10 mM methyl-β-cyclodextrin (MβCD) (Sigma-Aldrich) for 30 min at 37°C (Prior et al., 2003; Roy et al., 1999).

    Imaging:

    Article Title: Differential Ras activation between caveolae/raft and non-raft microdomains.
    Article Snippet: .. Cell culture, transfection, and imaging COS cells were transfected with pRaichu-tH or pRaichu-tK using the Lipofectin method (Gibco BRL). .. For disruption of caveolae/ rafts, cells were incubated in Hank’s Balanced Salt Solution (HBSS) containing 10 mM methyl-β-cyclodextrin (MβCD) (Sigma-Aldrich) for 30 min at 37°C (Prior et al., 2003; Roy et al., 1999).

    Modification:

    Article Title: Bromodomain analysis of Brd2-dependent transcriptional activation of cyclin A
    Article Snippet: .. Low passage NIH/3T3 cells (A.T.C.C., Manassas, VA, U.S.A.) were grown in DMEM (Dulbecco's modified Eagle's medium; Invitrogen) supplemented with 10% normal calf serum and PSG (penicillin, streptomycin and glutamine), maintained at less than 75% confluence and transfected in triplicate in 60 mm dishes at 50% confluence with plasmids by the Lipofectin® method (Invitrogen), then harvested at 100% confluence after 24–36 h. Transfections contained 3.0 μg of DNA/dish, comprised of 1.0 μg of Brd2 construct, 0.5 μg of cyclin A promoter luciferase reporter and 0.5 μg of Ki-ras expression vector, and were balanced with empty pcDNA(I) vector (Invitrogen). .. Normalization of luciferase activity was initially performed with reference to units of expressed β-galactosidase from a co-transfected vector and, after high reproducibility had been established, performed against protein concentration in the cell lysates.

    Construct:

    Article Title: Bromodomain analysis of Brd2-dependent transcriptional activation of cyclin A
    Article Snippet: .. Low passage NIH/3T3 cells (A.T.C.C., Manassas, VA, U.S.A.) were grown in DMEM (Dulbecco's modified Eagle's medium; Invitrogen) supplemented with 10% normal calf serum and PSG (penicillin, streptomycin and glutamine), maintained at less than 75% confluence and transfected in triplicate in 60 mm dishes at 50% confluence with plasmids by the Lipofectin® method (Invitrogen), then harvested at 100% confluence after 24–36 h. Transfections contained 3.0 μg of DNA/dish, comprised of 1.0 μg of Brd2 construct, 0.5 μg of cyclin A promoter luciferase reporter and 0.5 μg of Ki-ras expression vector, and were balanced with empty pcDNA(I) vector (Invitrogen). .. Normalization of luciferase activity was initially performed with reference to units of expressed β-galactosidase from a co-transfected vector and, after high reproducibility had been established, performed against protein concentration in the cell lysates.

    Article Title: Tat-dependent repression of human immunodeficiency virus type 1 long terminal repeat promoter activity by fusion of cellular transcription factors.
    Article Snippet: Transcription initiation from HIV-1 long terminal repeat (LTR) promoter requires the virally encoded transactivator, Tat, and several cellular co-factors to accomplish the Tat-dependent processive transcription elongation.. Individual cellular transcription activators, LBP-1b and Oct-1, on the other hand, have been shown to inhibit LTR promoter activities probably via competitive binding against TFIID to the TATA-box in LTR promoter.. To explore the genetic interference strategies against the viral replication, we took advantage of the existence of the bipartite DNA binding domains and the repression domains of LBP-1b and Oct-1 factors to generate a chimeric transcription repressor.

    Luciferase:

    Article Title: Bromodomain analysis of Brd2-dependent transcriptional activation of cyclin A
    Article Snippet: .. Low passage NIH/3T3 cells (A.T.C.C., Manassas, VA, U.S.A.) were grown in DMEM (Dulbecco's modified Eagle's medium; Invitrogen) supplemented with 10% normal calf serum and PSG (penicillin, streptomycin and glutamine), maintained at less than 75% confluence and transfected in triplicate in 60 mm dishes at 50% confluence with plasmids by the Lipofectin® method (Invitrogen), then harvested at 100% confluence after 24–36 h. Transfections contained 3.0 μg of DNA/dish, comprised of 1.0 μg of Brd2 construct, 0.5 μg of cyclin A promoter luciferase reporter and 0.5 μg of Ki-ras expression vector, and were balanced with empty pcDNA(I) vector (Invitrogen). .. Normalization of luciferase activity was initially performed with reference to units of expressed β-galactosidase from a co-transfected vector and, after high reproducibility had been established, performed against protein concentration in the cell lysates.

    Expressing:

    Article Title: Bromodomain analysis of Brd2-dependent transcriptional activation of cyclin A
    Article Snippet: .. Low passage NIH/3T3 cells (A.T.C.C., Manassas, VA, U.S.A.) were grown in DMEM (Dulbecco's modified Eagle's medium; Invitrogen) supplemented with 10% normal calf serum and PSG (penicillin, streptomycin and glutamine), maintained at less than 75% confluence and transfected in triplicate in 60 mm dishes at 50% confluence with plasmids by the Lipofectin® method (Invitrogen), then harvested at 100% confluence after 24–36 h. Transfections contained 3.0 μg of DNA/dish, comprised of 1.0 μg of Brd2 construct, 0.5 μg of cyclin A promoter luciferase reporter and 0.5 μg of Ki-ras expression vector, and were balanced with empty pcDNA(I) vector (Invitrogen). .. Normalization of luciferase activity was initially performed with reference to units of expressed β-galactosidase from a co-transfected vector and, after high reproducibility had been established, performed against protein concentration in the cell lysates.

    Article Title: Tat-dependent repression of human immunodeficiency virus type 1 long terminal repeat promoter activity by fusion of cellular transcription factors.
    Article Snippet: Transcription initiation from HIV-1 long terminal repeat (LTR) promoter requires the virally encoded transactivator, Tat, and several cellular co-factors to accomplish the Tat-dependent processive transcription elongation.. Individual cellular transcription activators, LBP-1b and Oct-1, on the other hand, have been shown to inhibit LTR promoter activities probably via competitive binding against TFIID to the TATA-box in LTR promoter.. To explore the genetic interference strategies against the viral replication, we took advantage of the existence of the bipartite DNA binding domains and the repression domains of LBP-1b and Oct-1 factors to generate a chimeric transcription repressor.

    Incubation:

    Article Title: A novel double-enhanced suicide gene therapy in a colon cancer cell line mediated by gef and apoptin.
    Article Snippet: Background Double-suicide gene therapy is a promising strategy for the treatment of advanced cancer.. It has become an important research line in the development of gene therapy to overcome the drawbacks of single-gene therapy.. Objective The aim of this study was to investigate the usefulness of double-suicide gene therapy with the two suicide genes, gef and apoptin, in colon carcinoma.

    Selection:

    Article Title: A novel double-enhanced suicide gene therapy in a colon cancer cell line mediated by gef and apoptin.
    Article Snippet: Background Double-suicide gene therapy is a promising strategy for the treatment of advanced cancer.. It has become an important research line in the development of gene therapy to overcome the drawbacks of single-gene therapy.. Objective The aim of this study was to investigate the usefulness of double-suicide gene therapy with the two suicide genes, gef and apoptin, in colon carcinoma.



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